A simple, accurate, precise and sensitive reverse phase liquid chromatography (RP-HPLC) methods was developed and validated for simultaneous estimation of chlorthalidone (CLT) and olmesartan medoxomil (OLM) in tablet dosage form using a Enable C18 (250 mm, -4.6 mm, 5 �¼m particle size) column and methanol: acetonitrile: water (60:20:20 v/v) as a mobile phase at a flow rate of 1.0 ml/min with detection wavelength 220 nm. The retention time for OLM and CLT was obtained as 1.89�±0.1 min and 2.98�±0.2 min respectively. The linearity was found in the range of 6.25-37.50 �¼g/ml and 10-60 �¼g/ml for CLT and OLM respectively. The developed method was validated as per ICH guideline, for its accuracy, precision, limit of detection and limit of quantitation.
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